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KMID : 0604220070140010029
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Korean Journal Investigative Dermatology
2007 Volume.14 No. 1 p.29 ~ p.36
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The Effect of Retinoic Acid on Expression of Human Beta Defensin-2 and LL-37 in Keratinocyte
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Kim Yeon-Jin
Seo Seong-Jun
Hong Chang-Kwun
Ahn Ji-Young
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Abstract
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Human skin is able to mount a fast response against harmful injury through the rapid production of inducible antimicrobial peptide (AMP) such as the human β-defensins (hBD), cathelicidin-family termed LL-37. Keratinocyte secrete AMPs upon stimulation with exogenous factor such as lipopolysaccharides (LPS), UV radiation and proinflammatory cytokines-tumor necrosis factor (TNF)-α and interleukin (IL)-1. In psoriasis patients, antimicrobial peptides tend to be expressed abundantly in the epidermal layer of the skin. Retinoic acid (RA) modulates immunological and inflammatory response and it also affects epidermal cell growth and differentiation by activation the retinoid acid receptor. RA is effective and safe in the treatment of psoriasis. This study aimed to investigated the hBD-2 and LL-37 expressions of human keratinocytes after exposure to the RA. Keratinocytes were cultured and to assess the expression of defensin-2 and LL 37 in the control group that was not treated with any stimulants, the experiment group treated with LPS, TNF-α, or UVB each, the experiment group treated again with RA, reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting, and immunohistochemical stainings were performed. Based on our experiment results, it was found that hBD2 and LL-37 expression had been regulated by stimulants-UV, LPS, TNF-α and RA in keratinocyte. We found retinoic acid can down-regulate both hBD and LL-37.
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KEYWORD
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Human beta defensin-2, LL-37, Retinoic acid, Keratinocyte
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